Hepatitis E huaketo IgM ELISA Kit

Whakaahuatanga Poto:

Hepatitis E huaketo IgM Ripene Whakamātautau ka whakamahia mo te rapunga tohu o te Hepatitis E Virus IgM antibody i roto i te serum tangata, plasma (EDTA, heparin, sodium citrate) toto katoa ranei (EDTA, heparin, sodium citrate).Ko te whakamatautau ka whakamahia hei awhina i te tohu mate mate kakā viral, na te Hepatitis E Virus.

Ko te huaketo Hepatitis E (HEV) he wheori RNA kore-kopaki, he whenu kotahi ka tukuna i te tuatahi ma te ara fecal-waha, te whakaheke toto me te maternal-fetal pea.Ko te mate o te mate HEV ka puta he mate ate mate whakapeka me te mate urutaa, he mate ate whakapeka, he mate iti ranei e rite ana ki te Hepatitis A. Ahakoa e wha nga momo momo momo HEV, kotahi noa te serotype.

Ko te mate HEV i roto i te tangata ka whakaputa i nga paturopi IgM, IgA me IgG.Ko te pai o te HEV-IgM me te HEV-IgA he tohu o te mate HEV whakapeka, tata ranei.Ahakoa he pai te anti-HEV-IgM me te anti-HEV-IgA mo tetahi, e rua ranei, he tohu enei mo te mate HEV tata nei.Ko te aroaro o te mate HEV tata, me te mahi ate, ka taea te whakamahi ki te whakatau mehemea he mate whakapeka, he tata ranei te mate.Ko te noho o te mate HEV i roto i te ate ka taea te whakamahi ki te whakatau mena he mate kakā E te mate, he whakaora mai ranei i te mate hepatitis E.

Tukuna he imeera ki a matou

Taipitopito Hua

Tohu Hua

Tikanga

Ka kitea e tenei kete te mate hepatitis E huaketo IgM antibody (HEV-IgM) i roto i te serum tangata, tauira plasma ranei, kua pania i mua i nga takai moroiti polystyrene ki nga paturopi kua tukuna ki nga pūmua immunoglobulin M tangata (anti-μ mekameka).I muri i te taapiri tuatahi i nga tauira serum, plasma ranei hei tirotirohia, ka taea te hopu i nga paturopi IgM i roto i te tauira, me etahi atu waahanga kore (tae atu ki nga paturopi IgG motuhake) ka tangohia ma te horoi.I roto i te taahiraa tuarua, ko te HRP (horseradish peroxidase) -ko nga antigens kua honoa ka tino tauhohe me nga parepare HEV IgM anake.I muri i te horoinga ki te tango i te HRP-conjugate kore i herea, ka taapirihia nga otinga chromogen ki roto i nga puna.I te aroaro o (anti-μ) - (HEV-IgM) - (HEV Ag-HRP) immunocomplex, i muri i te horoinga o te pereti, ka tapirihia te TMB substrate mo te whanaketanga tae, a ko te HRP hono ki te matatini ka whakakorikori i te tauhohenga kaiwhakawhanake tae ki hangaia te matū kahurangi, tāpirihia te 50μl o Stop Solution, ka huri kōwhai.Ko te waahi o te whakauru o te antibody HEV-IgM i roto i te tauira i whakatauhia e te kaipanui microplate.

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Whakatakotoranga Hua

Tikanga	Ko te whakamatautau immunosorbent e hono ana i te Enzyme
Momo	Tikanga hopu
Tiwhikete	CE
Tauira	Serum tangata / plasma
Whakatakotoranga	48T / 96T
Te pāmahana rokiroki	2-8 ℃
Te ora papa	12 marama