Toxoplasma IgG ELISA Kit
Tikanga
Ka kitea e tenei kete te Toxoplasma lgG antibody (TOX-lgG) i roto i te serum tangata, i nga tauira plasma ranei, kua whakakikoruatia ki te Toxoplasma antigen te polystyrene microwell.I muri i te taapiri tuatahi i nga tauira serum, plasma ranei hei tirotirohia, ko nga paturopi motuhake e rite ana (TOX-lgG-Ab & etahi lgM-Ab) kei roto i nga tauira turoro ka herea ki nga antigens i te wahanga totoka, ka tangohia etahi atu waahanga kaore i herea ma te horoi.I te taahiraa tuarua, ko te HRP(horseradish peroxidase)-conjugated anti-human lgG ka taurite noa ki te tox lgG antibodies.I muri i te horoinga ki te tango i te HRP-conjugate kore i herea, ka taapirihia nga otinga chromogen ki roto i nga puna.I te aroaro o (TOX Ag) - (TOX-lgG) - (anti-tangata lgG-HRP) immunocomplex, i muri i te horoinga o te pereti, ka tapirihia te TMB substrate mo te whanaketanga tae, a ko te HRP hono ki te matatini ka whakakorikori i te tauhohenga kaiwhakawhanake tae. hei whakaputa i te matū kahurangi, tāpirihia te 50 µ I o Stop Solution, ka huri kōwhai.Ko te waahi o te whakaurunga o te antibody TOX-lgG i roto i te tauira i whakatauhia e te panui microplate.
Nga waahanga hua
Te tairongo teitei, te motuhake me te pumau
Whakatakotoranga Hua
Tikanga | Ko te whakamatautau immunosorbent e hono ana i te Enzyme |
Momo | Tikanga autaki |
Tiwhikete | NMPA |
Tauira | Serum tangata / plasma |
Whakatakotoranga | 48T / 96T |
Te pāmahana rokiroki | 2-8 ℃ |
Te ora papa | 12 marama |
Nga korero ota
ingoa hua | Puke | Tauira |
Toxoplasma IgG ELISA Kit | 48T / 96T | Serum tangata / plasma |